Research and Development – Philippine Institute of Pure and Applied Chemistry

A vital part of PIPAC’s package of services is basic and applied research conducted independently or in cooperation with client companies.

Institute Personnel and their Research Interests

- - Armando Victor M. Guidote, Jr., Ph.D.
    Research Interests:
    Organic Chemistry, Natural Products, Chemistry Education
    Click here to see the list of his publications.
  - Armando Jerome H. de Jesus, Jr., Ph.D.
    Research Interests:
    Molecular dynamics simulations, Membrane-protein interactions, Membrane protein-small molecule interactions
    Click here to see the list of his publications.
  - Ronaldo M. Fabicon, Ph.D.
    Research Interests:
    Industrial Chemistry
    Click here to see the list of his publications.
  - Crisanto M. Lopez, Dr.rer.nat
    Research Interests:
    Infection Biology, Fungal Biotechnology
    Click here to see the list of his publications.
  - Giselle Grace F. Lim-Co Yu Kang, Ph.D
    Research Interests:
    Biochemistry, Enzymes, Analytical Chemistry
    Click here to see the list of her publications.
  - Gilbert U. Yu, D.Eng
    Research Interests:
    Materials Science (Polymers and Supramolecules), Chemical Education, Development of Lab Experiment Modules
    Click here to see the list of his publications.
  - Ian Ken D. Dimzon, Ph.D
    Research Interests:
    Applied Analytical Chemistry, Mass Spectrometry, Chemical Metrology
    Click here to see the list of his publications.
  - Anna Carissa M. San Esteban, Ph.D
    Research Interests:
    Electrochemistry (corrosion, electrocatalytic processes); Coordination Chemistry (coordination polymers, metal-organic frameworks)
    Click here to see the list of her publications.

Selected Publications

Show all

2009

Agnes T. Aranas; Armando M. Guidote Jr.; Joselito P. Quirino

Sweeping and new on-line sample preconcentration techniques in capillary electrophoresis Journal Article

In: Analytical and Bioanalytical Chemistry, vol. 394, pp. 175-185, 2009.

Abstract | Links | Tags: analyte focusing by micelle collapse, capillary electrophoresis, on-line sample preconcentration, sample stacking, sweeping

@article{Aranas2009,

title = {Sweeping and new on-line sample preconcentration techniques in capillary electrophoresis},

author = {Agnes T. Aranas and Armando M. Guidote Jr. and Joselito P. Quirino},

url = {https://link.springer.com/article/10.1007/s00216-009-2646-7},

doi = {10.1007/s00216-009-2646-7},

year  = {2009},

date = {2009-03-03},

journal = {Analytical and Bioanalytical Chemistry},

volume = {394},

pages = {175-185},

abstract = {Sweeping is a powerful on-line sample preconcentration technique that improves the concentration sensitivity of capillary electrophoresis (CE). This approach is designed to focus the analyte into narrow bands within the capillary, thereby increasing the sample volume that can be injected, without any loss of CE efficiency. It utilizes the interactions between an additive [i.e., a pseudostationary phase (PS) or complexing agent] in the separation buffer and the sample in a matrix that is devoid of the additive used. The accumulation occurs due to chromatographic partitioning, complexation or any interaction between analytes and the additive through electrophoresis. The extent of the preconcentration is dependent on the strength of interaction involved. Both charged and neutral analytes can be preconcentrated. Remarkable improvements—up to several thousandfold—in detection sensitivity have been achieved. This suggests that sweeping is a superior and general approach to on-line sample preconcentration in CE. The focusing mechanism of sweeping under different experimental conditions and its combination with other on-line preconcentration techniques are discussed in this review. The recently introduced techniques of transient trapping (tr-trapping) and analyte focusing by micelle collapse (AFMC) as well as other novel approaches to on-line sample preconcentration are also described.},

keywords = {analyte focusing by micelle collapse, capillary electrophoresis, on-line sample preconcentration, sample stacking, sweeping},

pubstate = {published},

tppubtype = {article}

}